Molecular basis of RNA-dependent RNA polymerase II activity Coursework

Molecular basis of RNA-dependent RNA polymerase II natural action - Coursework ExampleThe RdRP activity has also been implicated in the replication of the RNA Genomes of HDV virus and plant viriods.3) What is meant by HDV? Why would investigators indigence to study HDV? In your answer, tell me about HDV and why investigators would want to study HDV. What is the ANTIGENOME? Why is it important in the experimental design?HDV is Hepatitis Delta Virus. The investigators have taken HDV for the study as RdRP activity of Pol II has been complete to be involved in the replication of the viral genome. Antigenome is the complementary positive RNA which serves as the usher for the subtraction of the negative strand of the virus. To understand the physiological significance of the RdRP activity, the terminal segment of the HDV antigenome, which directs RNA synthesis and is sensitive to the Pol II inhibitor alpha- amanitin, was studied.4) What is meant by FAM-labeled RNA products were separa ted by denaturing gelatin electrophoresis? DO NOT give me cookbook recipes, i.e. do not provide a step-by-step how to that could be employ to perform an experiment. Describe the method in general terms.FAM is 6 carboxy- flourescein label. Nucleotides labeled with such flourophores, have shown ameliorate sensitivity, versatility and normalization when separated by denaturing gel electrophoresis. After purification of the nucleotides the fluorescein tag was attached by incubation with the 5 terminal of the nucleotides.5) DATA INTERPRETATION Evaluate Figure 1. Break the analysis down. endeavour to understand what each lane of the gel represents. Make note of the controls as well. Your focus for your response is the fraction of the regard labeled b, c, and d. Use part a in assisting you. Summarize what each part (b, c, and d) of the figure suggests.The RNA synthesized by the RdRP activity of Pol II is fluorescent labeled and separated by gel electrophoresis and viewed. Lane 1 in t he gel shows only the reactant RNA.